FIG. 2.

OROBOROS O2k validation. (A) Modifications to OROBOROS O2k system for engineered bundle respirometry measurements. A Viton O-ring was added to the O2k closed chambers consisting of a glass reservoir, stopper with drug port, electrode, and magnetic stir bar. The O-ring provided a myobundle resting platform during magnetic stirring. (B) FCCP-induced maximal respiration at both atmospheric and hyperbaric oxygen tensions is not statistically different. Results represent mean ± SEM of five independent experiments and two separate donors. (C) Basal and maximal oxygen consumption rates remain unchanged when stir rate is at maximum speed (900 rpm). Results represent mean ± SEM of five independent experiments. FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone.