FIG. 5.

Complex I inhibition by rotenone dose dependently inhibits oxygen consumption rates of engineered muscle. (A) Basal oxygen consumption rates of human myobundles in the presence of increasing concentrations (0–1000 nM) of rotenone. Results represent mean ± SEM of four donors normalized to the vehicle control. (B) Relative fold changes of basal, leak, and maximal respiration states due to rotenone normalized to the no treatment control. (C) Bioenergetic health index calculated from the non-normalized respiration states in (B) is demonstrated. Results represent mean ± SEM of four to five donors. *p < 0.05, **p < 0.01, ^#p < 0.001.