FIG. 6.

Comparison of 2D and 3D cocultures. Seven-day expansion cultures were initiated with 6 × 10³ CD34⁺ cells each (10 CD34⁺ cells per spheroid), and increasing numbers of MSCs, as indicated on the x-axis. (A) Total hematopoietic cell fold expansion. (B) Total CD34⁺ cell culture yield. (C) Total CD34⁺CD38⁻ cell culture yield. (D) Total MSC fold expansion. Five replicate data sets from independent experiments are shown in each caption. Points on the lines represent the mean ± SD for four replicate wells from a single study. Cultures from the same study are connected by a line. SD, standard deviation. Statistical significance for each comparison was performed using ANOVA, with significance indicators as described in the Methods and Materials/Statistical analyses section.