FIG. 7.

Characterization of cell yield in 2D and 3D cultures without MSCs to highlight effect of PDMS microwells on CD38. (A) Total cell expansion was similar in 2D and 3D cultures. (B) CD34⁺ cell yield was similar in 2D and 3D cultures. (C) CD38⁻ cell yield was statistically greater in 3D PDMS microwell cultures. (D) CD34⁺CD38⁻ cell yield was statistically greater in 3D PDMS microwell cultures. All cell number data were generated using flow cytometry, as described in the Materials and Methods section. Three replicate experiments are shown. For each experiment, bars represent the mean ± SD for four replicate wells. Statistical significance for each comparison was performed using t-test, with significance indicators as described in the Methods and Materials/Statistical analyses section.