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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Cell Chem Biol. 2016 Nov 23;23(12):1539–1549. doi: 10.1016/j.chembiol.2016.10.014

Figure 4. Application of fluorescent biosensor to detect 2′, 3′-cGAMP in mammalian cell extracts.

Figure 4

(A) Schematic representation of the experimental procedure for cell extract analysis using the fluorescent biosensor. (B, C) Fluorescence readings for different concentrations of L929 (B) or HEK293T (C) cell extracts (DNA- or mock-stimulated). (D) Fluorescence readings for L929 cell extracts (DNA- or mock-stimulated) treated with active or denatured SVPD prior to biosensor addition. (E) Standard curve of fluorescence readings from mock-stimulated L929 cell extracts doped with known concentrations of 2′, 3′-cGAMP (black circles). Average fluorescence reading from DNA-stimulated L929 cell extracts is shown in open circle. Error bars indicate standard deviations for three (B, C) or two (D, E) independent biological replicates. P-values are calculated from student’s t-test comparison: **, P < 0.01; ***, P < 0.001, n.s, not significant, P > 0.05.