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CDK9 IRIF co-localize with BRCA1 at damaged DNA sites. MCF7 cells were exposed to IR (10 Gy) and recovered for 3 hours. Immunostaining was performed using (A) only anti-CDK9 or (B) anti-CDK9 and anti-BRCA1 antibodies. Insets depict the nucleus in lower magnification. ((C)- upper panel) Quantification of CDK9 positive nuclei and ((C)- lower panel) CDK9/BRCA1 co-localization.NT, not treated. Scale bars = 10 μm.
