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YopJ-mediated protein acetylation at the serine and lysine residues in HeLa cells. (a) Western blotting of HeLa cell lysates with transiently expressed YopJ (Y) or control (V). The blots were detected with the anti-flag monoclonal antibody and stained with IRDye800CW-conjugated rabbit anti-mouse IgG. M, low molecular weight protein marker. (b) MS-MS spectrum of a Sac peptide from protein coffilin-1. (c) MS-MS spectrum of a Kac peptide from RBBP4.
