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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Hepatology. 2017 Mar 22;65(5):1526–1542. doi: 10.1002/hep.29021

Figure 7. Loss of tm6sf2 perturbs intestinal clearance of dietary lipid.

Figure 7

Representative TEM images of ultrathin 70 nm sections from enterocytes in anterior intestine of larvae injected with control or tm6sf2_e4 MO treated with (A) high fat meal (HFD) for 3 hours or (B) a 3 hour HFD followed by 18 hour clearance. CLD, cytosolic lipid droplet. BB, brush border (C) Quantification of enterocyte CLD accumulation in control MO or tm6sf2_e4 MO larvae treated with a 3 hour HFD followed by 18- or 24-hour clearance. Data calculated average CLD area relative to total cell area measured in individual cells on images in which at least three contiguous cells with visible nuclei could be visualized, n=4–12 larvae. ** p<0.01 relative to control MO at same condition. # p<0.01 relative to control MO 3hr HFD; & p<0.05 relative to tm6sf2 MO 3-hr HFD.. (D) Quantification of the number of enterocyte cytosolic lipid droplet per cell in control MO or tm6sf2_e4 MO larvae treated a 3 hour HFD followed by 18- or 24-hour clearance. n=4–12 larvae. *** p<0.001 relative to control MO at same condition. & p<0.05 relative to tm6sf2 MO 3hr HFD.