Fig. 1.

Otophylloside (Ot B) extended lifespan and improved thermo-tolerance in a C. elegans model of AD. a Chemical structure of Ot B. b Survival curves of CL2006 animals treated with vehicle or 50 μM of Ot B. c Survival percentage of CL2006 animals in heat resistance experiments. Heat resistance experiments were carried out at 35 °C and calculated by 2 h. d Survival percentage of CL2006 animals in heat resistance recovery experiment. The experiments were carried out at 35 °C for 7 h, then transferred to 16 °C and the number of dead worms was measured after 24 and 48 h. All the assays were carried out in triplicate, and at least three independent trials were performed. The columns showed the mean value of three independent experiments with error bars representing SEM. *** represents p < 0.001, * represents p < 0.05, calculated using two-tailed t test. Statistical details and repetition of this experiment are summarized in Table S1, S2 (Supplementary information)