Table 1.
Primers used in this study.
| Primer | Sequence 5′ → 3′ | Purpose |
|---|---|---|
| 16S Uni F | AGAGTTTGATCCTGGCTCAG | Species identification |
| 16S Uni R | ACGGMTACCTTGTTACGACTT | Species identification |
| DENF | GAAAGAAGCGCGCACCGACAG | Species identification |
| PARF | GAAGCATTGGAAGCGAAAGTTTCG | Species identification |
| ROGF | ATTGCAGAAGATGTAACAGTAAGC | Species identification |
| ATYF | TCTCTTGTTGAAGAATTAGAACGC | Species identification |
| DISF | AACGCGTTGAAATTCGTCATGAAC | Species identification |
| VR | GTGTAACAAGGGAGTACGGACC | Species identification |
| pBS F | CAGCAATAAACCAGCCAGCC | pBSJL1 detection |
| pBS R | CGCCGCATACACTATTCTCAG | pBSJL1 detection |
| TetM F | AGTAAAATGCAGGCGAGTGAAG | recA & CGL mutagenesis |
| TetM R | CCCAGGACACAATATCCACTTG | recA & CGL mutagenesis |
| gspE up F3 | GTCGTCTTATGGGGAATGAAGG | CGL mutagenesis |
| Tmp up R3 |
CTTCACTCGCCTGCATTTTACT ACACCACAACAGGAACAAGC |
CGL mutagenesis |
| 4715 dn F |
CAAGTGGATATTGTGTCCTGGG GCAAGGGGGCTTAAATGGATTG |
CGL mutagenesis |
| 4725 dn R | TCTATTTCTATACGGCGTGAAGG | CGL mutagenesis |
| gspE up F4 | GGCTGTCAGATGGCAGTTATTC | CGL mutagenesis |
| 4725 dn R2 | GGACCACCACCACGTTG | CGL mutagenesis |
| gspF dn F3 | GGACCACCACCACGTTG | CGL mutagenesis |
| pulO dn R3 | TCCCCTCCAAATCAACTGATAGAG | CGL mutagenesis |
| gspE up F3 | GTCGTCTTATGGGGAATGAAGG | CGL mutagenesis |
| gspF dn F2 | AAGTACATCGTTGGCTTTGGC | CGL mutagenesis |
| pulO dn R2 |
CTTCACTCGCCTGCATTTTACT GTCCCTTTCATACCTTGTCCTT |
CGL mutagenesis |
| pulO dn F2 |
CAAGTGGATATTGTGTCCTGGG GTGTTGGTTCTCTCTATCTCTTACTAAC |
CGL mutagenesis |
| 964 dn R | CCTTCATATCAAAATCGTGAGGATCA | CGL mutagenesis |
| recA up F | AGTACTGGTGGGTTAGGACC | recA mutagenesis |
| recA up R |
CTTCACTCGCCTGCATTTTACT CAACGCTGCTTGTCTACCATC |
recA mutagenesis |
| recA dn F |
CAAGTGGATATTGTGTCCTGGG CCGCTGAATTTGATTTGATGTATGG |
recA mutagenesis |
| recA dn R | CAGCCTCTTGCTCAGCTTG | recA mutagenesis |
| recA up F2 | CGCTCAAGATACAACAAGTTATGGC | recA mutagenesis |
| recA dn R2 | ACGAGCCACATCAACACCG | recA mutagenesis |
Sequences in bold are complementary to the tetM tetracycline resistance cassette.