Figure 1.

Verification of PCR primer specificity and efficiency for each endogenous plasmid in B. burgdorferi strain 297. PCR products were separated on 3% metaphor agarose gel. Each product for linear (A) and circular (B) plasmids with corresponding designation were labeled on top. The mixed product from pooled primer mix for linear plasmids (mixLP) and circular plasmids (mixCP) were on the right of each gel, and the band corresponding to each plasmid is labeled with designated letter on the right. DNA ladder is labeled on the left. ^*The primer sequences for lp25(E) were based on bptA sequence.