Table 2.
PCR primer sequences for each linear plasmid.
| Linear plasmid | Sequence 5′->3′ | Length (bp) | Coordination 5′–3′ | Tm (°C) | Concentration in 12.5 × primer mix (uM) | Amplicon size (bp) |
|---|---|---|---|---|---|---|
| lp25F* | CGTTATCTACCGTTTATAGGTTTGA | 25 | 266–290 | 52.5 | 2.7 | 100 |
| lp25R* | TTGAAACCTTAGCATCTTCAAATCCTT | 27 | 367–341 | 55.3 | 2.7 | |
| lp54F | TCTTAATATCAACCTAGAATATTCC | 25 | 965–989 | 48.0 | 2.7 | 125 |
| lp54R | TAACAGACGAAGAAGAAGAGACTTT | 25 | 1,089–1,065 | 53.7 | 2.7 | |
| lp28-6F | ATTGATGAATGGCGTTACCATTAGT | 25 | 11,486–11,510 | 54.9 | 2.25 | 151 |
| lp28-6R | CATTGTATCAGACATAACACTTCAT | 25 | 11,636–11,612 | 51.2 | 2.25 | |
| lp28-4F | CTTGCGATCTACCAACAATGAGTAA | 25 | 27–51 | 55.1 | 2.7 | 175 |
| lp28-4R | AGTTTATCTGATATTAGGAGATAGT | 25 | 201–177 | 48.5 | 2.7 | |
| lp28-1F | TCAATCAAACATATTGGGTGAAGAA | 25 | 53,00–5,324 | 53.0 | 2.25 | 200 |
| lp28-1R | TTGCCTGTATTGCTAAATTACTATG | 25 | 5,499–5,475 | 51.5 | 2.25 | |
| lp28-3F | TGTCTAAGGAAGGTTTAAGGCTTAT | 25 | 21,131–21,155 | 53.8 | 2.25 | 225 |
| lp28-3R | AGAAATGCAGTGCTTGCGTCTAAAT | 25 | 21,355–21,331 | 57.6 | 2.25 | |
| lp28-5F | TTAGGAACTCTGACTATCATGGAAT | 25 | 5,175–5,199 | 53.2 | 2.25 | 251 |
| lp28-5R | AAGGCGTCATTACAATTATCTAAACA | 26 | 5,425–5,400 | 53.0 | 2.25 | |
| lp17F | AGCGAAGAATTATTCTTGCAATGTG | 25 | 12,507–12,531 | 54.4 | 2.25 | 275 |
| lp17R | CGACTTCTTATATAGCTGAGATTCT | 25 | 12,781–12,757 | 51.7 | 2.25 | |
| lp38F | AATCCAGGTATTCTTGTTGCTGGTC | 25 | 20,896–20,920 | 57.2 | 3.38 | 325 |
| lp38R | TTATTAGGAGACGATATTAATATAA | 25 | 21,220–21,196 | 45.0 | 3.38 | |
| lp36F | AAGTGGTGAATTGGAGGAGCCTATT | 25 | 15,394–15,418 | 58.2 | 2.25 | 369 |
| lp36R | TTAGCAAAGTTGTCAAGGCGTGTAGA | 26 | 15,762–15,737 | 58.5 | 2.25 |
*
The primer sequences for lp25 were based on bptA gene sequence.