Table 3.
PCR primer sequences for each circular plasmid.
| Circular plasmid | Sequence 5′->3′ | Length(bp) | Coordination 5′–3′ | Tm (°C) | Concentration in 12.5 × primer mix (uM) | Amplicon size (bp) |
|---|---|---|---|---|---|---|
| cp32-4F | TGAGCAGCACAAGTAGATGATGCTT | 25 | 27,800–27,824 | 58.6 | 2.38 | 121 |
| cp32-4R | CCGGGGATAATGCTAGTCAACAA | 23 | 27,920–27,898 | 56.8 | 2.38 | |
| cp32-5F | AAGGTGCTTTAGACACAAGAGATGTGA | 27 | 20,972–20,998 | 58.0 | 2.38 | 153 |
| cp32-5R | AATTGTCTTGTATAGATTCCAACTTC | 26 | 21,124–21,099 | 51.5 | 2.38 | |
| cp32-1F | AAACATTAGTAGAAAGTGAATTTGATTTAC | 30 | 21,109–21,138 | 51.5 | 2.38 | 176 |
| cp32-1R | CCTATGTTCCTTATAAGGCAAGGGC | 25 | 21,284–21,260 | 57.3 | 2.38 | |
| cp32-6F | TGGAGATATTAATGGGGTGGCAATT | 25 | 28156–28180 | 56.5 | 2.38 | 197 |
| cp32-6R | CCAACAAGCTATTCCCTTCTACAAT | 25 | 28,352–28,328 | 55.0 | 2.38 | |
| cp32-7F | TTCAATGAATCCGGATGATGTTGA | 24 | 18,535–18,558 | 54.7 | 2.38 | 225 |
| cp32-7R | TTATTGTTTAATGCTGTTATATATGC | 26 | 18,759–18,734 | 48.5 | 2.38 | |
| cp32-3F | CCATTTATATTCTTAAAGTCGTTTA | 25 | 16,474–16,498 | 47.5 | 3.57 | 251 |
| cp32-3R | TGATCATCACCGCCTTGATCTAAAG | 25 | 16,724–16,700 | 56.8 | 3.57 | |
| cp32-12F | TTTGTATCCTTATCTGTATAACCAT | 25 | 16,720–16,744 | 49.6 | 2.38 | 295 |
| cp32-12R | TTGTATCAATGTTATTTGTAATGGC | 25 | 17,014–16,990 | 50.3 | 2.38 | |
| cp32-9F | GTATTCTAAGGGACTTAGATAAGT | 24 | 1,341–1,364 | 49.1 | 2.38 | 341 |
| cp32-9R | TGAATACTCTCAGCACTATTGACCT | 25 | 1,681–1,657 | 55.3 | 2.38 | |
| cp32-11F | GTTGTTGCCATTATTTGATTTACAG | 25 | 27,718–27,742 | 51.5 | 2.38 | 375 |
| cp32-11R | GAAATTTGTATTGCCTGTGGAGTTA | 25 | 28,092–28,068 | 53.8 | 2.38 | |
| cp26F | GGACAATTGGAACGTATCACACAGTA | 26 | 7,094–7,119 | 56.8 | 1.58 | 398 |
| cp26R | TTAAGGCTCTCACAGGAGGCTCCAT | 25 | 7,491–7,467 | 61.4 | 1.58 |