Table 3.
SRM detection of preproghrelin-derived peptides in the different chromatographic fractions (30, 40, and 60% Acetonitrile) in the stomach and duodenum of ghrl+/+ mice.
| Tissue | LC Fraction (%age acetonitrile) | Acyl ghrelin | Desacyl ghrelin | Obestatin-NH2 | Obestatin-Gly | des(1–10) -obestatin |
|---|---|---|---|---|---|---|
| Stomach | 30 | − | + | − | − | − |
| 40 | + | − | − | − | − | |
| 60 | + | + | + | − | − | |
| Duodenum | 30 | − | − | − | − | − |
| 40 | − | − | − | − | − | |
| 60 | + | − | + | − | − |
Acyl ghrelin and obestatin-NH2 were detected in the stomach and duodenum whereas, desacyl ghrelin was only detected in the stomach LC fractions of ghrl+/+ mice. MS-MS analyses further confirmed that obestatin-immunoreactivity in tissues is specific to the amidated peptide but not to obestatin-Gly nor des(1–10)-obestatin.