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. 2017 Apr 20;8:672. doi: 10.3389/fmicb.2017.00672

Figure 1.

Figure 1

ATMUV exhibits mild pathogenicity in mule ducklings following intramuscular injection. Twenty five 7-day-old healthy mule ducklings were challenged by intramuscular injection with 4.0 × 105 ELD50 of ATMUV in a volume of 0.4 mL per duckling. Ten same old ducklings were inoculated with 0.4 mL sterile PBS per duckling as a control. Three randomly selected ducklings were sacrificed at 0, 12, 24, 48, and 72 hpi, respectively. (A) Their organ tissues were harvested for detection viral infection by IFA. (C) The sera and (B) spleen homogenates of the ducklings at 0–72 hpi were prepared for detection the viral titer by TCID50 assay in DEF cells. (D) Five randomly selected ducklings in infected group and control group, respectively, were monitored rectal temperatures daily.