Table 1.

Effects of forage preparation on total gas, methane, and substrate disappearance in the RUSITEC fermentation.

				P-value
	Freeze-dried and ground	Frozen minced	SEM	Preparation	Day	Preparation × Day
Total gas, mL/d	1708.5	1758.6	144.59	0.82	0.05	0.66
Total gas, mL/g DM	170.0	179.0	10.13	0.55	<0.01	0.21
Total gas, mL/g DMD	193.6	204.0	11.54	0.55	<0.01	0.23
CH4, %	7.3	7.4	0.46	0.88	0.44	0.85
CH4, mg/d	90.8	106.0	5.54	0.09	0.10	0.46
CH4, mg/g DM	9.0	10.3	0.54	0.13	0.10	0.47
CH4, mg/g DMD	10.3	11.7	0.68	0.20	0.15	0.63
Forage IVDMD, %	91.1	92.3	0.67	0.24	0.02	0.12
Concentrate IVDMD, %	84.5	83.2	0.64	0.19	0.07	0.02

SEM, standard error of mean; DM, dry matter; DMD, dry matter disappearance; IVDMD, in vitro dry matter disappearance.