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. 2017 Apr 20;8:696. doi: 10.3389/fmicb.2017.00696

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Copyright © 2017 Mathur, Fallico, O’Connor, Rea, Cotter, Hill and Ross.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of 12 μM thuricin CD peptides on cell membrane integrity: (A) The probes Syto 9 and PI were used to evaluate effects on the membrane integrity of DPC6349 cells by adding one of the following: 12 μM Trnα; 12 μM Trnβ; 12 μM thuricin CD; pre-incubation with 1 μM Trnα followed by the addition of 12 μM Trnβ; cells pre-incubated with 1 μM Trnβ followed by the addition of 12 μM Trnα; PBS on its own (negative control). Live cell counts as quantified by FC at time points 0, 3, 5, and 21 h are shown. (B) Viable counts (cfu/ml) on LB agar plates showing recovery of DPC6349 cells exposed to the conditions described above. Asterisks indicate statistically significant decreases in cfu/ml counts for each sample, relative to cfu/ml counts at T0 for each respective sample. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001.