TABLE 1 .
Primer and probe sequences
| Agent | Gene target | Forward primer | Reverse primer | Probea |
|---|---|---|---|---|
| Borrelia burgdorferi | ospA | CCTTCAAGTACTCCAGATCCATTG | AACAAAGACGGCAAGTACGATC | FAM-CAACAGTAGACAAGCTTGA-MGB |
| Borrelia miyamotoi | flaB | AGCACAAGCTTCATGGACATTGA | GAGCTGCTTGAGCACCTTCTC | VIC-TGTGGGTGCAAATCAGGATGAAGCA-BHQ |
| Babesia microti | cox1 | CATCATGCCAGGCCTGTTTG | GAAGAAACCACAAGAGCAAATGC | Quasar 705-TACTACCCATACTGGTCGGTGCTCC-BHQ |
| Anaplasma phagocytophilum | 16S rRNA | GGCATGTAGGCGGTTCGGT | CACTAGGAATTCCGCTATCCTCTCC | Cy5-GCCAGGGCTTAACCCTGGAGCT-BHQ |
| Powassan virus | 3′-UTR | GTGATGTGGCAGCGCACC | CTGCGTCGGGAGCGACCA | Texas Red-CCTACTGCGGCAGCACACACAGTG-BHQ |
a
Abbreviations: FAM, 6-carboxyfluorescein; MGB, minor groove binder; BHQ, black hole quencher dye.