FIG 4 .

Sectored colony phenotype of RFP mutants. (A) Comparison of red, white, and sectored colony phenotype in visible and red fluorescent light. (B) Examples of various degrees of sectoring that range from mostly red to mostly white. (C) Red and white phenotypes in a sectored colony are genetically stable. A single sectored colony was restreaked on nonselective medium. Below is shown PCR analysis of full-length and rfpΔ33-403 from white “repaired” colonies isolated after transformation in the presence of a donor repair fragment.