Skip to main content
. 2017 Apr 7;12:776–786. doi: 10.1016/j.redox.2017.04.008

Fig. 4.

Fig. 4.

Increased expression of adhesion molecules after stimulation with CSEaq and pro-atherosclerotic low laminar flow. mRNA expression of endothelial (A) ICAM1 (intercellular adhesion molecule-1); (B) VCAM1 (vascular cell adhesion molecule-1); (C) SELE (selectin E); (D) CCL2 (MCP-1/monocyte-chemoattractant protein-1) after dose-dependent stimulation with CSEaq for 24 h under static conditions, n≥6. Flow-dependent regulation of (E) ICAM1; (F) VCAM1; (G) SELE and (H) CCL2 mRNA, n≥7. Primary human endothelial cells (HUVEC) were cultivated until confluence, stimulated with CSEaq in a concentration range from 10% to 50% for up to 24 h or subjected to laminar flow. Subsequently, mRNA expression was measured by real-time PCR. POLR2A was used as reference gene. Data are shown as mean (x-fold of time-matched controls (A–D) or basal controls (E–H)) ±SD. Time-matched controls are normalized to basal controls, where both are shown (E–H). *p<0.05 vs. time-matched controls (A–D) or basal controls (E–H), #p<0.05.