Table 1.
Primers used for analysis of human gene expression by real-time PCR.
| Target gene | Primer | Sequence (5´→3´) |
|---|---|---|
| POLR2A | Sense | ACCTGCGGTCCACGTTGTGT |
| Antisense | CCACCATTTCCCCGGGATGCG | |
| eNOS (NOS3) | Sense | GAACCTGTGTGACCCTCACC |
| Antisense | TGGCTAGCTGGTAACTGTGC | |
| NRF2 (NFE2L2) | Sense | CCCAATTCAGCCAGCCCAGC |
| Antisense | AACGGGAATGTCTGCGCCAA | |
| HMOX1 | Sense | CGGATGGAGCGTCCGCAACC |
| Antisense | TCACCAGCTTGAAGCCGTCTCG | |
| NQO1 | Sense | CCCCGGACTGCACCAGAGC |
| Antisense | CTGCAGCAGCCTCCTTCATGGC | |
| ICAM1 | Sense | ACCATGGAGCCAATTTCTCG |
| Antisense | GCGCCGGAAAGCTGTAGATG | |
| CCL2 (MCP-1) | Sense | GCTCAGCCAGATGCAATCA |
| Antisense | TTTGCTTGTCCAGGTGGTC | |
| VCAM1 | Sense | TGTGCCCACAGTAAGGCAGGC |
| Antisense | AGCTGGTAGACCCTCGCTGGA | |
| SELE | Sense | AGCCCAGAGCCTTCAGTGTA |
| Antisense | CTCCAATAGGGGAATGAGCA |
Abbreviations: POLR2A: RNA polymerase II subunit A; eNOS/NOS3: endothelial NO synthase; NRF2/NFE2L2: nuclear factor erythroid 2-related factor 2; HMOX1: heme oxygenase (decycling) 1; NQO1: NAD(P)H quinone dehydrogenase 1; ICAM1: intercellular adhesion molecule-1; CCL2: chemokine (C-C motif) ligand 2/MCP-1: monocyte-chemoattractant protein-1; VCAM1: vascular cell adhesion molecule-1; SELE: selectin E.