Figure 3.

Positional cloning of F29 and 1699-1. (A) Gentic linkage map of the F29 causative locus on the linkage group 1. LjT05K01 and LjT46E19 are TAC clones. DNA markers and their positions are indicated with the numbers of recombinants (in parentheses). (B) ORFs in the F29 locus delimited by recombination events and LjERN1 protein structure predicted by the nucleotide sequence are shown. The mutation site on F29 and the large deletion found in 1699-1 was indicated by a grey arrowhead and grey parentheses. (C and D) Complementation test. F29 hairy roots transformed with an ERN1 construct (C) and with an GUS vector as a negative control (D) were inoculated with M. loti for 32 days. Upper and bottom panels are bright field images and fluorescent images from a GFP transformation marker, respectively. Bars = 2 mm.