Fig. 5.

Design of net charge increasing mutation in the heavy chain. (a) Effect of all mutations on the global stability (ΔG) and stability of the complex between the heavy and light chain. Blue: Mutations that were not selected. Red: Selected mutations. (b) Left: Crystal structure of mAb2 in complex with VEGF. Gray: mAb2, green: VEGF. Top right: Zoom on the net charge increasing mutation SH21R. Red: SH21, blue: RH21. Bottom right: Zoom on the net charge increasing mutation SH85R. Red: SH85, blue: RH85. Images were made using YASARA Structure.