Table 3.
Antioxidant and aldose reductase inhibitory effects in rat lens of nine compounds isolated from the ethyl acetate fraction of Maackia amurensis.
| Isolated compounds | Antioxidant | RLAR inhibition | ||
|---|---|---|---|---|
| Quantitative reduction (%)a | IC50 (μM)b | Inhibition (%)c | IC50 (μM) | |
| Chlorogenic acid (1) | 17.09 | 27.23 ± 0.17 | 51.02 | 4.2 ± 3.20 |
| Formononetin-7-O-β-D-glucosyl [1–6] glucoside (2) | 1.04 | >500 | 2.32 | >500 |
| trans-Ferulic acid (3) | 20.06 | 13.51 ± 0.04 | 5.21 | >500 |
| Tectoridin (4) | 8.43 | >500 | 43.37 | 50.4 ± 2.17 |
| Piceatannol (5) | 34.68 | 6.73 ± 0.04 | 5.47 | >500 |
| Resveratrol (6) | 29.96 | 11.05 ± 0.04 | 29.31 | 117.6 ± 3.12 |
| Daidzein (7) | 0.48 | >500 | 28.14 | 151.9 ± 2.91 |
| Genistein (8) | 2.24 | >500 | 38.54 | 57.1 ± 3.23 |
| Formononetin (9) | 0.69 | >500 | 30.11 | 69.2 ± 2.57 |
| Troloxd | — | 16.83 ± 0.06 | — | — |
| Quercetine | — | — | — | 10.1 ± 2.18 |
aQuantitative reduction was defined as the amount of decrease in ABTS-offline HPLC.
bThe IC50 value was defined as the concentration of the 50% inhibition.
cInhibition was defined as the amount of efficacy in HPLC microfractionation.
dTrolox was used as positive control for ABTS assay.
eQuercetin was used as positive control for RLAR inhibitory activity measurement.