Table 2.
Immunolabeling Strategy
| Set | Antigen | Antibody |
Secondary antibody |
|---|---|---|---|
| Manufacturer, product number, host, dilution, fluorophore (if applicable) | Manufacturer, host, fluorophore, dilution | ||
| 1 | VGLUT1 | Abcam, ab104899, goat, 1:500 | Jackson ImmunoResearch, donkey, PE, 1:200 |
| VGAT | Synaptic Systems, 131011C2, mouse, 1:200, labeled with Oyster 488 by manufacturer | Not applicable | |
| Aβ42 | Synaptic Systems, 218703, rabbit, 1:250 | Jackson ImmunoResearch, donkey, Alexa-647 at 1:200 or PerCP at 1:10 | |
| PHF-tau | Thermo Fisher Scientific, MN1020, mouse, 1:500, labeled with Alexa 647 in house | Not applicable | |
| 2 | SNAP-25 | BioLegend, 836302, mouse, 1:10,000, labeled with PE in house | Not applicable |
| SERT | Sigma-Aldrich, SAB2500950, goat, 1:1000 | Jackson ImmunoResearch, donkey, PerCP, 1:10 | |
| Aβ42 | Synaptic Systems, 218703, rabbit, 1:250 | Jackson ImmunoResearch, donkey, Alexa-647 at 1:200 or PerCP at 1:10 | |
| 3 | VGLUT2 | Synaptic Systems, 135411, mouse, 1:500, labeled with PE in house | Not applicable |
| DAT | Abcam, ab5990, rat, 1:250 | Jackson ImmunoResearch, donkey, Alexa-488 labeled, 1:200 | |
| Aβ42 | Synaptic Systems, 218703, rabbit, 1:250 | Jackson ImmunoResearch, donkey, Alexa-647 at 1:200 or PerCP at 1:10 |
Aβ, amyloid β; DAT, dopamine transporter; PE, R-phycoerythrin; PerCP, peridinin chlorophyll protein; PHF, paired helical filament; SNAP-25, synaptosomal-associated protein 25; SERT, serotonin transporter; VGAT, vesicular GABA transporter; VGLUT, vesicular glutamate transporter.