Table 1.
Ex Vivo RV Intrinsic Contractile Function Parameters Evaluated by Isolated Perfused Hearts and Cardiac Mass (Heart, RV, and LV + Septum) at 3 and 6 Weeks after CTEPH
| Parameters | Sham | SU | PE | PE + SU | Comparison |
|---|---|---|---|---|---|
| 3 Weeks | |||||
| Developed pressure/RV weight | |||||
| RVEDP at 0 mmHg | 145 ± 14 | 151 ± 27 | 95 ± 41 | 51 ± 9 | P > 0.05 |
| RVEDP at 40 mmHg | 307 ± 12∗ | 299 ± 30∗ | 214 ± 37 | 128 ± 12† | P < 0.05 |
| dP/dtmax/RV weight | |||||
| RVEDP at 0 mmHg | 4231 ± 287 | 5087 ± 926∗ | 3101 ± 1313 | 1524 ± 260 | P < 0.05 |
| RVEDP at 40 mmHg | 5895 ± 170∗ | 5822 ± 746∗ | 3493 ± 1320 | 2282 ± 149 | P < 0.05 |
| RV weight (g) | 0.29 ± 0.02∗ | 0.30 ± 0.02∗ | 0.34 ± 0.01 | 0.62 ± 0.04† | P < 0.05 |
| LV + septum weight (g) | 1.23 ± 0.04 | 1.22 ± 0.01 | 1.27 ± 0.04 | 1.12 ± 0.08 | P > 0.05 |
| Heart weight (g) | 1.53 ± 0.04 | 1.53 ± 0.02 | 1.61 ± 0.04 | 1.75 ± 0.11 | P > 0.05 |
| RV/(LV + septum) ratio | 0.24 ± 0.01∗ | 0.24 ± 0.02∗ | 0.27 ± 0.01 | 0.55 ± 0.03† | P < 0.05 |
| 6 Weeks | |||||
| Developed pressure/RV weight | |||||
| RVEDP at 0 mmHg | 149 ± 12∗ | 127 ± 12∗ | 93 ± 46 | 57 ± 3 | P < 0.05 |
| RVEDP at 40 mmHg | 280 ± 11∗ | 258 ± 12∗ | 227 ± 52 | 126 ± 4† | P < 0.05 |
| dP/dtmax/RV weight | |||||
| RVEDP at 0 mmHg | 4699 ± 802∗ | 4093 ± 300∗ | 2593 ± 1160 | 1797 ± 135 | P < 0.05 |
| RVEDP at 40 mmHg | 3267 ± 1282∗ | 4836 ± 209∗ | 3267 ± 1282 | 2269 ± 119 | P < 0.05 |
| RV weight (g) | 0.30 ± 0.01∗ | 0.34 ± 0.01∗ | 0.33 ± 0.02 | 0.71 ± 0.03† | P < 0.05 |
| LV + septum weight (g) | 1.18 ± 0.04 | 1.26 ± 0.04 | 1.18 ± 0.04 | 1.17 ± 0.07 | P > 0.05 |
| Heart weight (g) | 1.49 ± 0.05∗ | 1.61 ± 0.06∗ | 1.52 ± 0.05 | 1.88 ± 0.09† | P < 0.05 |
| RV/(LV + septum) ratio | 0.25 ± 0.01∗ | 0.27 ± 0.02∗ | 0.28 ± 0.01 | 0.65 ± 0.06† | P < 0.05 |
RV-developed pressure and the maximum rate of increase in RV pressure during systole (dP/dtmax) were corrected for RV weights. Developed pressure/RVweight (mmHg/g) and dP/dtmax/RVweight (mmHg/s/g) were acquired at different RV end diastolic pressures (0 and 40 mmHg). RV/LV + septum ratio was used as a marker of RV hypertrophy. Values are means ± SEM. n = 4 to 6 per group.
CTEPH, chronic thromboembolic pulmonary hypertension; dP/dtmax, RV-developed pressure and maximum rate of pressure development; LV, left ventricular; PE, polystyrene microsphere; RV, right ventricular; RVEDP, RV end diastolic pressure; SU, tyrosine kinase inhibitor SU5416.
P < 0.05 versus PE + SU group.
P < 0.05 for PE versus PE + SU group.