Fig. 4.

Representative pyrogram images of KRAS sequencing a of codons 12 and 13 and b codons 60 and 61. Different cell lines harbor different KRAS gene mutations. a Various missense mutations (black arrow) are noted in codons 12 and 13 of AMCPAC cell lines; G12V (GGT → GTC) in AMCPAC01, G12D (GGT → GAT) in AMCPAC02 and AMCPAC03, G13D (GGC → GAC) in AMCPAC04, and AMCPAC06 G12R (GGT → CGT). Only AMCPAC05 shows no mutation in codon 12 and 13 (GGTGGC), but codon 60 and 61 mutation of AMCPCA cell lines b. In codons 60–61, GGTCAA sequence assays in reverse orientation as TTGCAA. Only AMCPAC05 has 2 mutations; insertion (white arrow) and missense mutation (black arrow) (GGTCAA → GGTCTACTA). While other cell lines show wile type KRAS. Arrows indicate KRAS mutation site in each cell lines, and among them white arrow is newly founded mutated site in this study