Skip to main content
. 2017 Apr 20;7:46480. doi: 10.1038/srep46480

Figure 3. Fluorescent images of sperm stained with DAPI DNA dye and anti-γH2AX antibody.

Figure 3

Human sperm were treated with anti-γH2AX antibody to detect double-strand breaks in DNA. Blue represents DAPI staining, indicating nuclear DNA in the head of the sperm, and red represents γH2AX staining. (A) Positive control. Sperm were treated with 1 mM H2O2 for 2 hours. The red areas overlaying the blue indicate the presence of double-stranded DNA breaks in the nucleus. (B) Experimental group. Sperm were exposed to 633 nm light for 30 minutes. There were only a few γH2AX foci seen in the sperm nuclei. (C) Negative control. There were little or no γH2AX foci seen in the sperm nuclei.