Table 2. Results of heart rate variability analysis in the frequency domain in the different sleep stages in RLS patients and healthy controls, and in RLS patients sorted according to the MEIS1 SNP rs2300478 genotype (GG or TT).

			N2	N3	REM
Control	LF	Median ± MAD	35.7 ± 11.6	35.4 ± 10.0	29.5 ± 8.5
	HF	Median ± MAD	25 ± 9.15	36.1 ± 15.7	18.9 ± 7.3
	LF/HF	Median ± MAD	1.81 ± 0.85	1.18 ± 0.71	1.82 ± 0.77
RLS	LF	Median ± MAD	43.3 ± 16.3	37.1 ± 10.8	27.2 ± 6.8
	HF	Median ± MAD	19.7 ± 10.3	25.7 ± 13.0	14.6 ± 7.2
	LF/HF	Median ± MAD	2.43 ± 1.42	1.47 ± 0.71	1.79 ± 0.77
Non adjusted	LF	p	0.002***	0.44	0.52
	HF	p	0.07	0.015*	0.57
	LF/HF	p	0.004***	0.17	0.09
Adjusted	LF	pa	0.059	0.71	0.64
	HF	pa	0.81	0.57	0.8
	LF/HF	pa	0.09	0.58	0.12
RLS TT	LF	Median ± MAD	39.9 ± 11.9	36.7 ± 11.9	28.1 ± 6.2
	HF	Median ± MAD	21.9 ± 12.1	27.3 ± 10.4	16.4 ± 8.7
	LF/HF	Median ± MAD	1.94 ± 0.95	1.35 ± 0.68	1.69 ± 0.65
RLS GG	LF	Median ± MAD	59.3 ± 15.8	38.6 ± 8.0	26.9 ± 2.5
	HF	Median ± MAD	14.4 ± 7.8	13.2 ± 10.0	13.2 ± 4.9
	LF/HF	Median ± MAD	4.00 ± 2.41	1.99 ± 1.09	1.88 ± 1.05
	LF	p	0.13	0.78	0.43
	HF	p	0.18	0.02*	0.09
	LF/HF	p	0.18	0.05*	0.13

LF = Low Frequency; HF = High Frequency. Data are presented as Median ± MAD. p = p value following Student or Wilcoxon’s test; p_a = p value adjusted for age and gender, or PLMS. *p ≤ 0.05; ***p ≤ 0.005.