Fig. 4.

Effect of iNOS dimerization inhibitor BBS-1 on iNOS expression. HEK 293 cells stably expressing iNOS (A) or RAW264.7 cells stimulated to produce iNOS by incubation in media containing LPS (100 ng/ml) and IFN-γ (10 units/ml) (B) were incubated for 18 h in the presence of BBS-1 (0–100 μM). A representative Western blot of cells lysates (50 μg/lane), using an anti-iNOS antibody, is shown (Upper). UD, presence of UD of iNOS. iNOS activity was evaluated by measuring nitrite accumulation in culture media (Lower) (means ± SD, n = 2).