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. 2017 Apr 20;7:46597. doi: 10.1038/srep46597

Figure 2. Co-expression of endogenous Flk1 and Nano-lantern driven by the Flk1 regulatory sequence in vascular ECs.

Figure 2

Co-expression of Venus and the endogenous Flk1 protein in Flk1-Nano-lantern BAC Tg embryos at 9.5 dpc. Tg embryos were subjected to immunohistochemical analysis with anti-GFP and anti-Flk1 antibodies. *Indicates strong autofluorescence caused by circulating blood cells. Circulating blood cells localized in blood vessels are also shown by arrows. Open arrowheads and a closed arrowhead indicate intersomitic vessels and the vitelline vein, respectively. Scale bars: 100 μm.