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. 2017 Apr 20;7:46580. doi: 10.1038/srep46580

Figure 3. The effect of a high salt concentration on the induction of IFNγ inducible chemokines in HK2 cells.

Figure 3

(A) qRT-PCR analyses evaluating mRNA expressions of each CXCLs in HK2 cells (n = 4). The cells were treated by IFNγ (10 ng/mL) for 36 h after a 2 h exposure of culture medium supplemented with either NaCl (80 mM) or Sorbitol (160 mM, as an osmotic control). A high salt concentration suppressed mRNA levels of all CXCLs induced by IFNγ, compared to the control. (B) ELISA analyses evaluating protein concentrations of each CXCLs released to culture supernatant from HK2 cells (n = 6). The cells were treated by IFNγ (10 ng/mL) for 72 h after a 2 h exposure of culture medium supplemented with either NaCl (80 mM) or sorbitol (160 mM, as an osmotic control). A high salt concentration also suppressed protein concentrations of CXCL9 and CXCL10 induced by IFNγ, compared to the control. Values are expressed as mean ± standard error of the mean (SEM). *p < 0.05; **p < 0.01.