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. 2017 Apr 20;7:46580. doi: 10.1038/srep46580

Figure 7. The essential involvement of the JAK1-STAT1 signaling pathway in the inductions of the IFNγ inducible chemokines.

Figure 7

The cells were treated by a JAK1 and JAK2 specific inhibitor, ruxolitinib (Ruxo) (1 μM), 2 h before the 36 h of IFNγ (10 ng/mL) treatment. (A) Left, Representative immunoblotting performed to evaluate the phosphorylation of STAT1 in the nucleus; Right, Densitometry analysis of the immunoblotting of the phosphorylation of STAT1 in the nucleus (n = 4). Full-length western blot images are presented in Supplementary Figure S6. Ruxo inhibited phosphorylation of STAT1 at tyrosine 701 in the nucleus. (B) qRT-PCR analyses were performed to evaluate CXCLs expression (n = 4). The expressions of CXCLs were markedly suppressed and no difference between the high salt concentration condition and control condition was evident.