Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 20;7:46324. doi: 10.1038/srep46324

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Specific markers show the purity of the subcellular fractions: voltage-dependent anion channel (VDAC) for mitochondria (Mit); glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for cytosol (Cyto), histone 1 for nucleus (Nu). (b) Representative subcellular distribution of VCP and iNOS in WT and VCP TG mouse hearts. GAPDH was used as a marker to exclude the contamination of cytosal in mitochondrial fraction. (c) Immunoblot of the expression of VCP and iNOS in mitochondrial fraction in TG and WT mouse hearts. VDAC was used as loading control of mitochondria. Negative staining of GAPDH and histone1 confirm the purity of mitochondrial fractions. (d) Ratio of VCP/VDAC and iNOS/VDAC in mitochondria in VCP TG mice compared to WT. n = 7/group. *p < 0.01 vs WT. (e) iNOS activity in isolated mitochondria from mouse hearts. n = 5/group. *p = 0.02 vs WT. Data are the mean ± SEM.