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. 2017 Apr 20;7:46667. doi: 10.1038/srep46667

Figure 6. Effect of SUV39H1 knock down and overexpression on the inflammation response of human small airway epithelial cells.

Figure 6

(a) Western blot analysis of SUV39H1 or H3K9me3 in normal small airway epithelial cells. HSAEpCs were transfected with SUV39H1 or control scramble siRNA. The SUV39H1 and H3K9me3 levels were reduced via siRNA transfection.(b) Cell supernatants were monitored for IL-8 secretion using an ELISA. The IL-8 and IL-6 levels were significantly increased in HSAEpCs knocked down with siSUV compared with siRNA control(siC). However, the IL-4 and IL-10 levels were not affected after transfection.(c) COPD HSAEpCs were transfected with SUV39H1(pSUV) or empty control vectors(pCMV). The SUV39H1 and H3K9me3 levels were increased via SUV39H1 expression vector transfection.(d) The IL-8 and IL-6 levels were significantly diminished in COPD HSAEpCs via SUV39H1 overexpression. There was no difference in the IL-4 and IL-10 levels between SUV39H1 overexpression and control cells. All data are presented as the mean ± SEM of at least three independent experiments. *p < 0.05 and **p < 0.01.