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. 2017 Apr 20;7:46659. doi: 10.1038/srep46659

Figure 2. The anti-EGFR aptamer blocks the endothelial trans-differentiation of BT-549 cells.

Figure 2

(a) BT-549 cells, seeded on Matrigel in the presence of 200 nmol/l CL4 or CL4Sc for 24 hours, were stained with anti-VE-cadherin antibody, visualized by fluorescence microscopy and photographed; nuclei were stained with DAPI. Magnification 20×, scale bar = 100 μm. (b–f) BT-549 cells were left untreated or treated as in (a) and mRNA levels of the indicated VM-genes were determined by RT-qPCR. Bars depict means ± SD of three independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05; one-way ANOVA followed by Tukey’s multiple comparison test. No statistically significant variations among CL4Sc- and mock-treatment was obtained.