Figure 1.

Drosophila pupal neurons with mutant IP₃R exhibit increased spontaneous Ca²⁺ signals and reduced store-operated Ca²⁺ entry (SOCE). (A,B) Traces showing spontaneous Ca²⁺ signals in individual pupal neurons in absence and presence of extracellular Ca²⁺ in wild type and itpr mutant respectively. (C) Mean spontaneous Ca²⁺ signals in itpr mutant (S224F/G1891S) neurons in presence of La and Co with extracellular Ca²⁺. (D) Mean spontaneous Ca²⁺ signals in wild type and itpr mutant neurons. (E) Mean Ca²⁺ signals in wild type and itpr mutant neurons due to store depletion with thapsigargin followed by SOCE. (F) Ca²⁺ signals for SOCE after subtracting spontaneous Ca²⁺ influx from Ca²⁺ influx induced by store-Ca²⁺ depletion with thapsigargin (mean data obtained from several separate experiments with same time intervals for recording). N > 100 cells.