Figure 4.

Neuronal knock-down of VGCCs does not affect Drosophila flight. (A) Flight time (mean ± SEM) with knockdown of VGCCs respectively in the mentioned neurons, cac (104186), Ca-β (102188), Ca-α1D (51491) and Ca-α1T (48008, 31961), N > 30 individual flies, P > 0.05, Kruskal-Wallis test for variance followed by Wilcoxon post hoc test. (B) Representative images showing store-depletion by thapsigargin and SOCE in TH positive pupal neurons in the indicated genotypes. (C) Box plot representing peak ΔF/F₀ with individual values for single neurons, mean, median and 25–75 quartiles for spontaneous Ca²⁺ influx, thapsigargin induced store depletion and SOCE respectively, N > 50 cells, P > 0.01, Kruskal-Wallis test for variance followed by Wilcoxon post hoc test. **P < 0.01.