Fig. 1.
Total RNA accumulations in HeLa cells infected with WT and ΔUL41 mutant virus. (A) HeLa cells were either mock-infected or infected with 10 pfu of HSV-1(F) or ΔUL41 mutant viruses per cell for 3 h. Transcription was then stopped by addition of 5 μg of actinomycin D (Act. D) per ml. In parallel, an identical series was incubated in medium containing 0.5% DMSO (control). The cells were harvested at the time intervals shown; total mRNA was extracted, and amounts of GAPDH, β-actin, IEX-1, and GADD45β transcripts were determined by Northern blotting. The ethidium bromide staining of total RNA samples is shown as a loading control. (B) HeLa cells were either mock-infected or exposed to 10 pfu of ΔUL41 repaired virus (ΔUL41R) per cell and incubated for 3 h. The cells were treated as described above, and total RNA was analyzed for GAPDH mRNA. The ethidium bromide staining of total RNA samples is shown as a loading control. The signal in the mock lane in A for β-actin reflects the unexpected interaction of the probe with size markers. In other experiments, e.g., Fig. 2, the size marker was run in a lane distant from the mock lane.