Fig. 2.
Cytoplasmic RNA accumulations in HeLa cells infected with WT and ΔUL41 mutant virus. HeLa cells were either mock-infected or exposed to 10 pfu of HSV-1(F) or ΔUL41 mutant virus per cell and incubated for 3 h. Transcription was then stopped by addition of 5 μg of actinomycin D (Act. D) per ml. In parallel, an identical series was incubated in medium containing 0.5% DMSO (control). The cells were harvested at the time intervals shown; cytoplasmic mRNA was extracted, and the amounts of GAPDH, β-actin, TTP, IEX-1, and GADD45β mRNAs were determined by Northern blotting. The ethidium bromide staining of cytoplasmic RNA samples is shown as a loading control.