Fig. 5.
Total and cytoplasmic β2- and β5-tubulin mRNA accumulations in HSV-1(F)- and ΔUL41-infected cells. HeLa cells were either mock-infected or infected with 10 pfu of HSV-1(F) or ΔUL41 mutant virus per cell and incubated for 3 h. Transcription was then stopped by addition of 5 μg of actinomycin D (Act. D) per ml. In parallel, an identical series was incubated in medium containing 0.5% DMSO (control). The cells were harvested, and total (A) or cytoplasmic (B) mRNAs were extracted at the time intervals shown. The amounts of β2- and β5-tubulin transcripts were determined by Northern blotting. The ethidium bromide staining of total and cytoplasmic RNA is shown as a loading control.