Fig. 1.

Expression of Aur-A gene (A) and protein (B) in untreated MGs, E₂-treated MGs, and E₂-induced MGTs. Representative individual samples of age-matched, untreated control (MC_1–2), 4.0-mo (ME4_1–2), and E₂-induced MGTs (MGT_1–2) were used. (A) Electrophoretic image of RT-PCR of Aur-A and β-actin mRNA, used as an internal control. RNA processing, primer details, and RT-PCR conditions are described in Materials and Methods. B Upper shows a Western blot of the relative expression of immunoprecipitated Aur-A in the same treatment groups. B Lower shows the Aur-A mRNA (▪) and protein (□) relative expression. The values represent the mean ± SE (n = 8).