Fig. 4.
Immunoblot analysis of the relative expression of γ-tubulin (A) and centrin (B) in untreated MGs, E2-treated MGs, and E2-induced MGTs. Whole-cell lysates from HeLa cells (HC), used as a positive control; age-matched untreated control, MC; 4-mo treated, ME41–2; and E2-induced MGT1–2 were prepared. (A) For γ-tubulin, 100-μg protein fractions were examined by Western analysis. (B) For centrin, 1,000-μg protein fractions were immunoprecipitated as described in Material and Methods. Lower shows the relative expression of γs-tubulin + γf-tubulin (▪) and centrin (□). The values represent the mean ± SE (n = 6).