Fig. 1.

Butyrate and SAHA induced apoptosis, caspase-3 activation, and cytochrome c release in HeLa cells. HeLa cells were harvested after 2 days of treatment with indicated concentrations of butyrate or SAHA. (A) Cell death was measured by Hoechst dye 33342 staining followed by flow cytometry as described. BuT, butyrate; Con, control. (B) Cytosolic proteins of each sample were assayed for caspase-3 activity as described. (C) Cytosolic proteins (40 μg) were subjected to immunoblotting against cytochrome c (Cyto). Immunoblotting against β-tubulin was performed as the loading control.