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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Nature. 2016 Sep 28;538(7623):99–103. doi: 10.1038/nature19766

Extended Data Figure 3. Characterization of prolonged TrkB activation and spine volume change during single spine sLTP.

Extended Data Figure 3

a, Prolonged time course of spine volume change following 2p-glutamate uncaging in rat hippocampal slices transfected with the TrkB sensor (TrkB) or mEGFP (GFP). n = 50/54 for TrkB sensor (9/10 for experiments longer than 20 min) and 8/8 for mEGFP (cells/spines).

b, Prolonged time course of TrkB activation in stimulated spines (Stim spine), the base of the spine neck (Spine base), adjacent spines (Adj spine), and the dendritic shaft adjacent to the stimulated spine (Dendrite). n = 50 cells with 54 Stim spine, Spine base, and Dendrite plus 59 Adj spine.

c-d, Time course (c) and quantification (d) of the transient (averaged over 1–2 min) and sustained (averaged over 20–40 min) phases of glutamate uncaging-induced spine volume change in rat hippocampal slices in the absence and presence of anisomycin (25 μM). n = 12/14 Ctrl and 5/5 anisomycin (cells/spines).

Data are mean +/− s.e.m.