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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Nature. 2016 Sep 28;538(7623):99–103. doi: 10.1038/nature19766

Extended Data Figure 8. Basal spine size and CaMKII activation in the presence and absence of post- synaptic BDNF.

Extended Data Figure 8

a-b, Quantification (a) and representative 2p images (f) of basal spine size/morphology in Bdnffl/fl slices transfected with EGFP (Cre Neg) or EGFP plus Cre (Cre Pos). n = 14/50 Cre Neg and 29/117 Cre Pos (cells/spines). Scale bar equates to 1μm.

c-d, Time course (c) and quantification (averaged over 0–45 s) (d) of CaMKII activation in Bdnffl/fl slices transfected with the CaMKII sensor (Cre Neg) or CaMKII plus Cre (Cre Pos). n = 7/13 Cre Neg and 7/15 for Cre Pos (cells/spines).

e-f, Time course and quantification of the transient phase of spine volume change for experiments in (c).

Data are mean +/− s.e.m. *p < 0.05 as determined by a two-tailed unpaired samples t-test.