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. Author manuscript; available in PMC: 2017 Apr 20.
Published in final edited form as: J Neurochem. 2015 Dec 10;136(3):510–525. doi: 10.1111/jnc.13391

Figure 1. Study Design.

Figure 1

Adult male Sprague-Dawley rats weighing 350–400 g were used for all experiments. Methamphetamine chloride (METH HCl) (4 × 10 mg/kg, i.p.) or saline (1 mL/kg) was administered to rats at 2-h intervals (solid red arrows). The animals were euthanized 3 days after the last METH or saline injection (solid black arrows) and assessed for the levels of tubulins and dopaminergic (DAergic) markers in the striatum and substantia nigra pars compacta (SNpc). A separate group of rats was treated with a microtubule-stabilizing drug epothilone D (EpoD) or vehicle 24 h before, during (after the 3rd injection) and 24 h after binge METH or saline treatment (purple arrows). Three 30-minute open-field motor activity measurements were performed for both groups at times indicated by dotted black arrows. The second group of animals was also euthanized at 3 days after the last METH or saline injection and assessed for striatal tubulins and DAergic markers.