Figure 1.

Effect of FtsZ2–1 overexpression before the onset of chloroplast division on the localization of chloroplast division proteins in synchronously cultured C. merolae. (A) Schematic diagram of the culture conditions. The control GFP heat-inducible (GFP) or FtsZ heat-inducible (FtsZ OX) cells were synchronized by a 12-h light/12-h dark cycle at 42°C and heat-shocked at 50°C at hour 8 (before the beginning of chloroplast division) for 2 h. (B) Immunofluorescent images of the control GFP and FtsZ OX cells showing the localization of FtsZ2–1, PDR1, and DRP5B at hour 16 in the synchronous culture. Green, immunostained FtsZ2–1, PDR1, or DRP5B; red, autofluorescence of the chloroplast; PC, phase-contrast. Scale bar = 1 μm. Two independent experiments produced similar results and the results from one experiment are shown.