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. 2017 Apr 20;37(2):BSR20160622. doi: 10.1042/BSR20160622

Figure 1. Cellular viability, apoptosis and CREB phosphorylation in the hypoxia-treated chondrocyte C28/I2 cells.

Figure 1

C28/I2 cells were inoculated under hypoxia or normoxia for 12, 24 or 48 h and were then analysed for the cellular viability with MTT assay, for the apoptosis with annexin V/FITC apoptosis detection kit, for the CASP 3 activity with AMC Caspase-3 Assay Kit and for the CREB phosphorylation with Western blotting assay. MTT assay results (A), Percentage of apoptotic cells (B), percentage of CASP 3 activity (C) of the C28/I2 cells under hypoxia or normoxia were presented respectively; (D-F) Western blotting assay of released Cyt c (E), cleaved CASP 3 (Clv-CASP 3) (E), CREB with p-CREB or without phosphorylation (CREB) (F) in the hypoxia-treated C28/I2 cells for 0, 12, 24 or 48 h. Each result was averaged for triple independent experiments. Statistical significance was shown as *P<0.05; **P<0.01 or ***P<0.001; ns, no significance.