Figure 5. LPA reduces the hypoxia-induced apoptosis in C28/I2 cells.

C28/I2 cells were treated with 0 or 10 nM LPA and then were incubated for 24 or 48 h under hypoxia, then the RhoA in protein level (A), the CREB with or without phosphorylation (B), the percentage of apoptotic cells (C) and the CASP 3 activity (D) were assayed respectively. All experiments were performed independently in triplicate. Statistical significance was shown as *P<0.05 or **P<0.01; ns: no significance.